$$Phenyl phosphate, is an artificial substrate and incubated with alkaline phosphatase in the presence of $4$mM homoarginine. The data when plotted is a Michaelis$-$Menton enzyme. The equation of a Lineweaver$-$Burk plot with inhibitor is y $=0\mathrm{.}7348$x $+1\mathrm{.}9194,$ while the equation of a straight line without inhibitor is y $=0\mathrm{.}7265$x $+0\mathrm{.}7224.$ The units for concentration is mM and the units for Vo are mM $*$ min$-1).$ Please calculate Km and Vmax for alkaline phosphatase with and without homoarginine. What would i do if ever given the wrong units for the Vo or Km$?$ How would you convert that into correct untis? Thanks! 

Answer rating: 100% (QA)

To calculate Km and Vmax using the LineweaverBurk plot or double reciprocal plot you can utilize the ... View the full answer