Beef catalase has been used to accelerate the decomposition of hydrogen per- oxide to yield water...
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Beef catalase has been used to accelerate the decomposition of hydrogen per- oxide to yield water and oxygen /Chem. Eng. Educ., 5, 141 (1971)]. The con- centration of hydrogen peroxide is given as a function of time for a reaction mixture with a pH of 6.76 maintained at 30°C. t (min) 10 20 50 100 0.0106 CH,0, (mol/L) 0.02 0.01775 0.0158 0.005 (a) Determine the Michaelis-Menten parameters Vmax and Km- (b) If the total enzyme concentration is tripled, what will the substrate con- centration be after 20 min? (c) How could you make this problem more difficult? Beef catalase has been used to accelerate the decomposition of hydrogen per- oxide to yield water and oxygen /Chem. Eng. Educ., 5, 141 (1971)]. The con- centration of hydrogen peroxide is given as a function of time for a reaction mixture with a pH of 6.76 maintained at 30°C. t (min) 10 20 50 100 0.0106 CH,0, (mol/L) 0.02 0.01775 0.0158 0.005 (a) Determine the Michaelis-Menten parameters Vmax and Km- (b) If the total enzyme concentration is tripled, what will the substrate con- centration be after 20 min? (c) How could you make this problem more difficult?
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