1. How long (in hours) would it take to replicate human chromosome 1 (2.8 X 10...
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1. How long (in hours) would it take to replicate human chromosome 1 (2.8 X 10 bp of DNA) if it contained a single replication origin located in the middle of the chromosome? (DNA replication in animal cells proceeds at about 150 bases per second for each replication complex containing a DNA polymerase molecule.) 2. Regarding the processes of transcription and translation 3'-GGCTATTACGTACGG-5' 5'-CCGATAATGCATGCC-3' A) RNA polymerase II is transcribing this DNA segment from right to left. List the nucleotide sequence of the mRNA produced in this process; be sure to label the 5' and 3' ends. B) The 40S subunit of the ribosome binds to the 5'-end of this mRNA and searches for an initiation codon to begin translation. Once the initiation codon has been found, the 60S subunit of the ribosome joins the 405 subunit and the mRNA is translated starting at the initiation codon. Provide the primary structure of the translation product (use 3-letter amino acid codes); be sure to label the amino terminus of your translation product. (A table of the Genetic Code is provided in Figure 18-10 of your textbook.) 3. Single base pair mutations occur at a rate of about one mutation per 10° base pairs per cell division in E. coll. You are studying a gene that is 2000 nucleotides in length and is not essential for cell replication and survival. In a population of one million E. coli cells how many cells on average will have a mutation in your gene of interest after one population doubling? 1. How long (in hours) would it take to replicate human chromosome 1 (2.8 X 10 bp of DNA) if it contained a single replication origin located in the middle of the chromosome? (DNA replication in animal cells proceeds at about 150 bases per second for each replication complex containing a DNA polymerase molecule.) 2. Regarding the processes of transcription and translation 3'-GGCTATTACGTACGG-5' 5'-CCGATAATGCATGCC-3' A) RNA polymerase II is transcribing this DNA segment from right to left. List the nucleotide sequence of the mRNA produced in this process; be sure to label the 5' and 3' ends. B) The 40S subunit of the ribosome binds to the 5'-end of this mRNA and searches for an initiation codon to begin translation. Once the initiation codon has been found, the 60S subunit of the ribosome joins the 405 subunit and the mRNA is translated starting at the initiation codon. Provide the primary structure of the translation product (use 3-letter amino acid codes); be sure to label the amino terminus of your translation product. (A table of the Genetic Code is provided in Figure 18-10 of your textbook.) 3. Single base pair mutations occur at a rate of about one mutation per 10° base pairs per cell division in E. coll. You are studying a gene that is 2000 nucleotides in length and is not essential for cell replication and survival. In a population of one million E. coli cells how many cells on average will have a mutation in your gene of interest after one population doubling?
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