Suppose that you want to sequence the following two DNA fragments: 

(1) 5'-GCGCAATTCTTC-primer site-3' 

(2) 3'-primer site -AACCGGTACTAC-5'

In each case you first use PCR to amplify the fragment, so that there is sufficient DNA for sequencing. You carry out Sanger dideoxy sequencing with a primer that anneals to the primer site, and then separate the products of the four sequencing reactions by gel electrophoresis. 

Draw the bands that should appear on the gel from the sequencing reactions for fragments (1) and (2) respectively.

 

Transcribed Image Text:

(1) Reaction containing ddATP ddCTP ddGTP ddTTP (2) Reaction containing ddATP ddCTP ddGTP ddTTP 3 (1) Reaction containing ddATP ddCTP ddGTP ddTTP (2) Reaction containing ddATP ddCTP ddGTP ddTTP 3

Answer rating: 100% (QA)

Answer the gel profile for both newly synthesized dna ... View the full answer