1. The figure to the right shows oxygen consumption following addition of a 300 - mitochondria...
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1. The figure to the right shows oxygen consumption following addition of a 300 - mitochondria preparation of mitochondria to 500 nmol ADP B-hydroxybutyrate dissolved in medium 200 (a) containing 70 mM sucrose, 220 mM ADP all mannitol, 2 mM HEPES buffer, 5 mM converted (c) to ATP magnesium chloride, 5 mM potassium 100 - phosphate, 1 mM EDTA, and 0.1% bovine serum album, pH 7.4 and at 25°C. Dissolved oxygen in the system is measured with an oxygen electrode. 0 1 2 3 4 5 6 minutes after adding mitochondria a. What sequence of reactions is initiated upon the addition of mitochondria to the system and results in the rapid consumption of dissolved oxygen as shown in (a) of the figure? b. What accounts for the slow rate of oxygen consumption observed in (b) of the figure? c. Suggest events that lead to the rapid oxygen consumption upon addition of 500 nmol of ADP to the system (part (c) of the figure). d. What accounts for the slow rate of oxygen consumption observed in (d) of the figure? e. In what form is oxygen consumed in parts (a) to (d) of the figure? f. How many moles of ATP are synthesized per oxygen atom consumed? 02 (nmol) 1. The figure to the right shows oxygen consumption following addition of a 300 - mitochondria preparation of mitochondria to 500 nmol ADP B-hydroxybutyrate dissolved in medium 200 (a) containing 70 mM sucrose, 220 mM ADP all mannitol, 2 mM HEPES buffer, 5 mM converted (c) to ATP magnesium chloride, 5 mM potassium 100 - phosphate, 1 mM EDTA, and 0.1% bovine serum album, pH 7.4 and at 25°C. Dissolved oxygen in the system is measured with an oxygen electrode. 0 1 2 3 4 5 6 minutes after adding mitochondria a. What sequence of reactions is initiated upon the addition of mitochondria to the system and results in the rapid consumption of dissolved oxygen as shown in (a) of the figure? b. What accounts for the slow rate of oxygen consumption observed in (b) of the figure? c. Suggest events that lead to the rapid oxygen consumption upon addition of 500 nmol of ADP to the system (part (c) of the figure). d. What accounts for the slow rate of oxygen consumption observed in (d) of the figure? e. In what form is oxygen consumed in parts (a) to (d) of the figure? f. How many moles of ATP are synthesized per oxygen atom consumed? 02 (nmol)
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Related Book For
Introduction to Probability and Statistics
ISBN: 978-1133103752
14th edition
Authors: William Mendenhall, Robert Beaver, Barbara Beaver
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