The following data were obtained from 3 separate enzyme kinetic experiments using 3 different substrates S1, S2
Question:
The following data were obtained from 3 separate enzyme kinetic experiments using 3 different substrates S1, S2 and S3 forming products P1, P2 and P3 respectively. The amount of enzyme in each reaction is 1 µM. Find out the rate and graph the data using a Michaelis-Menton and Lineweaver-Burk plots and determine the values for Km, Vmax, Kcat, and Kcat/Km. Which among the 3 substrate is best substrate for this enzyme and why?
[S] (mM) | [P1] (mM) in 60 min | [P2] (mM) in 240 min | [P3] (mM) in 30 min |
2.5 | 0.170 | 0.126 | 0.194 |
2 | 0.201 | 0.135 | 0.213 |
1.5 | 0.167 | 0.129 | 0.206 |
1 | 0.162 | 0.131 | 0.203 |
0.6 | 0.159 | 0.135 | 0.174 |
0.3 | 0.148 | 0.131 | 0.163 |
0.15 | 0.122 | 0.111 | 0.122 |
0.2 | 0.095 | 0.117 | 0.114 |
0.1 | 0.075 | 0.072 | 0.104 |
0.05 | 0.049 | 0.040 | 0.071 |
Intermediate Accounting
ISBN: 978-0324592375
17th Edition
Authors: James D. Stice, Earl K. Stice, Fred Skousen