The kinetics of an enzyme were analyzed in the absence of inhibitors, as well as in...
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The kinetics of an enzyme were analyzed in the absence of inhibitors, as well as in the presence of Inhibitor A and Inhibitor B. Using the given data below, construct or calculate the following (Make sure to label graphs with appropriate axes and equations, and circle final answers): [S] MM 3 6 12 24 36 No Inhibitor Vo (mmol/min) 3.90 6.00 8.40 10.8 12.0 Inhibitor A V₁ (mmol/min) 2.40 3.60 5.10 6.60 7.20 Inhibitor B V₂ (mmol/min) 1.29 2.40 3.90 6.30 8.55 a. Write out the Michaelis-Menten and Lineweaver-Burke equations Plot all of the data on one plot as Michaelis-Menten saturation curves c. Plot all of the data on one plot in the Lineweaver-Burk format b. d. Define Km and Vmax e. Mathematically determine the Km and Vmax in the absence and in the presence of inhibitors f. Determine what type of inhibitors A and B are The kinetics of an enzyme were analyzed in the absence of inhibitors, as well as in the presence of Inhibitor A and Inhibitor B. Using the given data below, construct or calculate the following (Make sure to label graphs with appropriate axes and equations, and circle final answers): [S] MM 3 6 12 24 36 No Inhibitor Vo (mmol/min) 3.90 6.00 8.40 10.8 12.0 Inhibitor A V₁ (mmol/min) 2.40 3.60 5.10 6.60 7.20 Inhibitor B V₂ (mmol/min) 1.29 2.40 3.90 6.30 8.55 a. Write out the Michaelis-Menten and Lineweaver-Burke equations Plot all of the data on one plot as Michaelis-Menten saturation curves c. Plot all of the data on one plot in the Lineweaver-Burk format b. d. Define Km and Vmax e. Mathematically determine the Km and Vmax in the absence and in the presence of inhibitors f. Determine what type of inhibitors A and B are
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Related Book For
Biochemistry Concepts and Connections
ISBN: 978-0321839923
1st edition
Authors: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
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