We wish to study the effect of an uncompetitive inhibitor on an isomerase. the tests are carried

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We wish to study the effect of an uncompetitive inhibitor on an isomerase. the tests are carried out without adding the inhibitor, yielding the following results:

subsequently the inhibitor is added, in such a way that the [ES] / [ESI] ratio is 0.25

Calculate:

a. Km, Vmax, Apparent Vmax

b. Predict how much the initial velocity decreases once the inhibitor is added, with a [S]= 3.5

Related Book For answer-question

Biochemistry Concepts and Connections

ISBN: 978-0321839923

1st edition

Authors: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews

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Posted Date: Sep 13, 2020 07:28 PM

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We wish to study the effect of an uncompetitive inhibitor on an isomerase. the tests are carried

Question:

[S] (MM) 0,5 3,5 Vo (mM/s) 23,5 44 [S] (MM) 0,5 3,5 Vo (mM/s) 23,5 44

Expert Answer:

a we calculate Kun And Vwux Apparent Vmax 05 M 235 Ms and ... View the full answer

Biochemistry Concepts and Connections

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