What is the equilibrium constant? How many moles of ligand bind per mole of riboflavin binding protein?
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What is the equilibrium constant? How many moles of ligand bind per mole of riboflavin binding protein? 0.040 mM of riboflavin binding protein was used.
Riboflavin (ligand) mM | Absorbance |
0.025 | 0.025 |
0.050 | 0.038 |
0.075 | 0.045 |
0.100 | 0.056 |
0.125 | 0.058 |
0.150 | 0.065 |
Km = 2 mM
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