You monitor the reaction of a protein forming a dimer and find an apparent rate constants of
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Question:
You monitor the reaction of a protein forming a dimer and find an apparent rate constants of 6 s-1 with 1 μM total protein and 10 s-1 with 5 μM total protein.
a. What are the forward and reverse rate constants for the dimerization reaction?
b. What is the standard Gibbs free energy change for this reaction at 300K?
c. Would the dimerization reaction be spontaneous under standard reaction conditions?
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