Determine the field gradient required to separate two polypeptides (D = 5 x 10 -6 cm 2
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Determine the field gradient required to separate two polypeptides (D = 5 x 10-6 cm2/s), with respective pI values of 4.2 and 4.8, using IEF with a linear field gradient of 0.3/cm. Use a standard criteria for IEF that peak-to-peak separation must be three times the average peak width.
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Solution Equation 78 can be rewritten as F GVA GVLTBM G LC1000 kmol A A S L S L TBM Plugging in the...View the full answer
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Related Book For
Separation Process Principles Chemical And Biochemical Principles
ISBN: 9780470481837
3rd Edition
Authors: By J. D. Seader, Ernest J. Henley, D. Keith Roper
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