Suppose you have just determined the DNA base sequence for an especially strong promoter in Escherichia coli and you are interested in incorporating this sequence into an expression vector. Describe the steps you would use. What precautions are necessary to be sure that this promoter actually works as expected in its new location?
Answer to relevant QuestionsMany genetic systems use the lacZ gene encoding β-galactosidase as a reporter. What advantages or problems would there be if (a) luciferase or (b) green fluorescent protein were used instead of β-galactosidase as ...What is evolution? What processes give rise to genetic variation? What processes can cause allele frequencies to change over time? What is a stromatolite and when can they be found in Earth’s history? What is homoplasy and why does it create problems for molecular phylogenetic analyses? Give an example of a fermentation that does not employ substrate-level phosphorylation. How is energy conserved in this fermentation?
Post your question