T7 DNA polymerase is less sensitive to dideoxynucleotides than the E. coli DNA polymerase I fragment described
Question:
(a) For a given concentration of dideoxynucleotides, which enzyme is more likely to give a longer "ladder" on the sequencing gel?
(b) Which enzyme is best used for sequencing DNA close to the primer?
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a T7 DNA polymerase is likely to give a longer ladder since it is ...View the full answer
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Related Book For 
Fundamentals of biochemistry Life at the Molecular Level
ISBN: 978-0470547847
4th edition
Authors: Donald Voet, Judith G. Voet, Charlotte W. Pratt
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