Take a look at solved problem SI. Let's suppose a male is heterozygous for two polymorphic sequence-tagged
Question:
Take a look at solved problem SI. Let's suppose a male is heterozygous for two polymorphic sequence-tagged sites. STS-1 exists in two sizes: 211 bp and 289 bp. STS-2 also exists in two sizes: 115 bp and 422 bp. A sample of sperm was collected from this man, and individual sperm were placed into 30 separate tubes. Into each of the 30 tubes were added the primers that amplify STS-1 and STS-2, and then the samples were subjected to PCR. The following results were obtained:
A. What is the arrangement of these STSs in this individual?
B. What is the linkage distance between STS-1 and STS-2?
C. Could this approach of analyzing a population of sperm be applied to RFLPs?
Transcribed Image Text:
12 34 5 6 7 8 9 1 422 bp 289 bp 211 bp 115 bp 11 12 13 14 15 1617 18 19 20 422 bp 289 bp 211 bp| 115bp 21 22 23 24 25 26 27 28 29 30 422 bp 289 bp 211 bp 15 bp
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