The catalytic efficiency of many enzymes depends on pH. Chymotrypsin shows a maximum value of kcat/KM at
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The change in k cat between pH 6 and pH 7 must involve loss ...View the full answer
Biochemistry Concepts and Connections
ISBN: 978-0321839923
1st edition
Authors: Dean R. Appling, Spencer J. Anthony Cahill, Christopher K. Mathews
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A substance that alters color in solution over a constrained range of pH values is known as a pH indicator or acid-base indicator. The indicator chemical just needs to alter color slightly in order to be noticed. Indicators work on the basis that they react with water to produce the hydrogen cation H+ or hydronium ion H3O+. The indicator molecule\\\'s color changes as a result of the reaction. Since indicators have distinct color ranges for color change, they can occasionally be combined to provide color changes over a wider pH range.
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