# Question: The following data are from Gennings Chinchilli and Carter 1989

The following data are from Gennings, Chinchilli, and Carter, (1989). An in vitro toxicity study of isolated hepatocyte suspensions was conducted to study the impact of combining carbon tetrachloride (CCl4) and chloroform (CHCl3) on the toxicity of cells. Cell toxicity was measured by the amount ofÂlactic dehydrogenase (LDH) enzyme leakage. The study involved randomly assigning four flasks to each of the 16 treatments obtained by combining four levels of CCl4 (0, 1.0, 2.5, and 5.0 mM) with four levels of CHCl3 (0, 5, 10, and 25 mM). The percentage of LDH leakage from the cells in each of the 64 flasks was measured just prior to applying the treatment to the flasks and at .01, .25, .5, 1, 2, and 3 hours after applying the treatment. The percentages of LDH leakage are given in the following table.

a. Plot the mean percentages of LDH leakage by time for the 16 treatments. Does there appear to be an effect due to increasing the level of CCl4 or CHCl3?

b. From the plot, does there appear to be an increase in the mean percentages of leakage as time after treatment increases?

c. Plot a profile plot of the mean percentage of LDH leakage separately for each time period. Does there appear to be a difference in the profile plots?

a. Plot the mean percentages of LDH leakage by time for the 16 treatments. Does there appear to be an effect due to increasing the level of CCl4 or CHCl3?

b. From the plot, does there appear to be an increase in the mean percentages of leakage as time after treatment increases?

c. Plot a profile plot of the mean percentage of LDH leakage separately for each time period. Does there appear to be a difference in the profile plots?

**View Solution:**## Answer to relevant Questions

Refer to Exercise 18.30. In Exercise 18.30 The following data are from Gennings, Chinchilli, and Carter, (1989). An in vitro toxicity study of isolated hepatocyte suspensions was conducted to study the impact of combining ...Sodium nitrate, a preservative that is used in some processed meats, such as bacon, jerky, and luncheon meats, could increase your heart disease risk. A consumer protection organization is evaluating the level of sodium ...An industrial engineer is studying the hand insertion of electronic components on printed circuit boards in order to improve the speed of the assembly operation. She has designed three assembly fixtures (F1, F2, and F3) and ...A horticulturist is interested in examining A horticulturist is interested in examining the yield potential of three A horticulturist is interested in examining the yield potential of three new varieties of asparagus. She ...Refer to Exercise 19.1. Use the least significant difference criterion to identify which pairs of methods of irrigation have significantly different mean yields. In Exercise 19.1 In Exercise 15.1, we described an experiment ...Post your question