The genes for both the a- and b-globin chains of hemoglobin contain introns (i.e., they are split genes). How would this fact affect your plans if you wanted to introduce the gene for a-globin into a bacterial plasmid and have the bacteria produce a-globin?
Answer to relevant QuestionsWhen proteins are separated using native gel electrophoresis, size, shape, and charge control their rate of migration on the gel. Why does DNA separate based on size, and why do we not worry much about shape or charge? What is the functional difference between regular PCR and qPCCR? How are DNA microarrays used to screen a patient’s cells for a cancer prognosis? Both ADA-SCID and type I diabetes are diseases based on lack of a particular protein. Why has the pioneering work on gene therapy focused on SCID instead of on diabetes? Explain the mode of action of the herpes viral RNAs and how they confound the immune system.
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