Question: The kinetic data in the following table were obtained for

The kinetic data in the following table were obtained for the reaction of carbon dioxide and water to produce bicarbonate and hydrogen ion catalyzed by carbonic anhydrase:
CO2 + H2O → HCO-3 + H+
[H. De Voe and G. B. Kistiakowsky, J. Am. Chem. Soc. 83, 274 (1961)]. From these data, determine KM and Vmax for the reaction.
Carbon Dioxide Concentration 1/Velocity
(mmol L -1 (M-1 sec)
1.25.............. 36 × 103
2.5............... 20 × 103
5.0............... 12 × 103
20.0.............. 6 × 103

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  • CreatedJuly 08, 2015
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