The M and H subunits of lactate dehydrogenase have very similar sizes and shapes but differ in amino acid composition. If the only difference between the two were that the H subunit had a glutamic acid in a position where the M subunit had a serine, how would the five isozymes of LDH separate on electrophoresis using a gel at pH 8.6? (See Chapter 5 for details on electrophoresis.)
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