Two biochemistry students are about to use mitochondria isolated from rat liver for an experiment on oxidative phosphorylation. The directions for the experiment specify addition of purified cytochrome c from any source to the reaction mixture. Why is the added cytochrome c needed? Why does the source not have to be the same as that of the mitochondria?
Answer to relevant QuestionsReflect and apply What are two advantages of the components of the electron transport chain being embedded in the inner mitochondrial membrane? Why is it difficult to determine an exact number for P/O ratios? Reflect and apply How does mitochondrial structure contribute to aerobic metabolism, particularly to the integration of the citric acid cycle and electron transport? It has been stated many times that fatty acids cannot yield a net gain in carbohydrates. Why can odd-chain fatty acids be thought to break this rule to a small extent? Draw a six-carbon saturated fatty acid and show where the double bond is created during the first step of b-oxidation. What is the orientation of this bond?
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