What are restriction enzymes? Why does the presence of a restriction enzyme in a cell not cause the degradation of that cell’s DNA?
Answer to relevant QuestionsHow does the insertional inactivation of β-galactosidase allow the presence of foreign DNA in a plasmid vector such as pUC19 to be detected? What are the essential characteristics of an artificial chromosome? What is the difference between a BAC and a YAC? Genetic engineering depends on vectors. Describe the properties needed in a well-designed plasmid cloning vector. Many genetic systems use the lacZ gene encoding β-galactosidase as a reporter. What advantages or problems would there be if (a) luciferase or (b) green fluorescent protein were used instead of β-galactosidase as ...How many bacterial species are there? Why do we not know this number more precisely?
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