What are the main procedures involved in a western blot?
Answer to relevant QuestionsWhere did western blot get its name? What is the purpose of the tag on the bait protein described in figure 5.26? What are two ways that a compound can be eluted from an ion-exchange column? What could be the advantages or disadvantages of each? An amino acid mixture consisting of lysine, leucine, and glutamic acid is to be separated by ion-exchange chromatography, using a cation-exchange resin at pH 3.5, with the eluting buffer at the same pH. Which of these amino ...The hydrolysis of a phenylalanine-containing peptide is catalyzed by a-chymotrypsin with the following results. Calculate KM and Vmax for the reaction. Peptide Concentration (M) Velocity (M min-1) 2.5 × ...
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