What are the major advantages of radioisotopic methods in the study of microbial ecology? What type of controls (discuss at least two) would you include in a radioisotopic experiment to show 14CO2 incorporation by phototrophic bacteria or to show 35SO42− reduction by sulfate-reducing bacteria?
Answer to relevant QuestionsHow might you combine SIP and NanoSIMS to identify novel methane-consuming cells in a natural community? Compare and contrast the use of FISH and phylochips for enumerating microbial cells in natural environments. What advantages and limitations do each of these methods have? Design a SIP experiment that would allow you to determine which organisms in a lake water sample were capable of oxidizing the hydrocarbon hexane (C6H14). Assume that four different species could do this. How would you ...The surface of a rock in a flowing stream will often contain a biofilm. What advantages could be conferred on bacteria growing in a biofilm compared with growth within the flowing stream? Why are most iron-oxidizing chemolithotrophs obligate aerobes and why are the better-studied iron oxidizers acidophilic?
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