What are two ways that a compound can be eluted from an ion-exchange column? What could be the advantages or disadvantages of each?
Answer to relevant QuestionsWhy do most people elute bound proteins from an ion-exchange column by raising the salt concentration instead of changing the pH? What could be an advantage of using an anion exchange column based on a quaternary amine [i.e., resin–N+ (CH2CH3)3] as opposed to a tertiary amine [resin–NH+ (CH2CH3)2]? Define steady state, and comment on the relevance of this concept to theories of enzyme reactivity. For the reaction of glucose with oxygen to produce carbon dioxide and water, Glucose + 6O2 → S 6CO2 + 6H2O The DG° is 22880 kJ mol –1, a strongly exergonic reaction. However, a sample of glucose can be maintained ...For the following aspartase reaction (see Question 28) in the presence of the inhibitor hydroxymethylaspartate, determine KM and whether the inhibition is competitive or noncompetitive.
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