What is an advantage of using an ELISA in-stead of a protein microarray to study a proteome? What is a disad-vantage?
Answer to relevant QuestionsWhat is the advantage to transferring proteins bands form a gel to nitrocellulose during the western blot? Give an example of a scenario in which you could partially isolate a protein with differential centrifugation using only one spin. How can gel-filtration chromatography be used to arrive at an estimate of the molecular weight of a protein? Would you use a pH meter to monitor the progress of the reaction described in Question 14? Why or why not? How do the KM values for glucoki-nase and hexokinase reflect their roles in sugar metabolism?
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