What is proteomics?
Answer to relevant QuestionsWhat is the purpose of the tag on the bait protein described in figure 5.26? What is the basis for the separation of proteins by the following techniques? (a) Gel-filtration chromatography (b) Affinity chromatography (c) Ion-exchange chromatography (d) Reverse phase HPLC Design an experiment to purify protein X on an anion-exchange column. Protein X has an isoelectric point of 7.0. Define steady state, and comment on the relevance of this concept to theories of enzyme reactivity. Distinguish between the molecular mechanisms of competitive and noncompetitive inhibition.
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