What is the advantage to transferring proteins bands form a gel to nitrocellulose during the western blot?
Answer to relevant QuestionsWhat is the point to labeling a secondary an-tibody with a marker that can be visualized instead of just labeling the primary antibody? You are purifying a protein for the first time. You have solubilized it with homogenization in a blender followed by differential centrifugation. You wish to try ammonium sulfate pre-cipitation as the next step. Knowing ...What is the main difference between reverse phase HPLC and standard ion-exchange or gel filtration chromatography? Distinguish between the lock-and-key and induced-fit models for binding of a substrate to an enzyme. If we describe an enzyme like aspartate transcarbamoylase and say that it exhibits cooperativity, what do we mean?
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