What is the basis for the technique called ELISA?
Answer to relevant QuestionsWhat are some ways that the antibody-protein complex can be visualized in ELISA or western blots? What is the point to labeling a secondary an-tibody with a marker that can be visualized instead of just labeling the primary antibody? What is the basis for the separation of proteins by the following techniques? (a) Gel-filtration chromatography (b) Affinity chromatography (c) Ion-exchange chromatography (d) Reverse phase HPLC What could be an advantage of using an anion exchange column based on a quaternary amine [i.e., resin–N+ (CH2CH3)3] as opposed to a tertiary amine [resin–NH+ (CH2CH3)2]? Determine the values of KM and Vmax for the de-carboxylation of a β-keto acid given the following data. Substrate Concentration (mol L–1) Velocity (mM ...
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