What is the purpose of the tag on the bait protein described in figure 5.26?
Answer to relevant QuestionsWhat are some of the assumptions behind the logic of the experiment described in figure 5.26? What are two ways that a compound can be eluted from an affinity column? What could be the advantages or disadvantages of each? What could be an advantage of using an anion exchange column based on a quaternary amine [i.e., resin–N+ (CH2CH3)3] as opposed to a tertiary amine [resin–NH+ (CH2CH3)2]? For an enzyme that displays Michaelis–Menten kinetics, what is the reaction velocity, V (as a percentage of Vmax), observed at the following values? (a) [S] 5 KM (b) [S] 5 0.5KM (c) [S] 5 0.1KM (d) [S] 5 2KM (e) [S] 5 10KM Where do lines intersect on a Lineweaver–Burk plot showing competitive inhibition? On a Lineweaver–Burk plot showing noncompetitive inhibition?
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