Where did western blot get its name?
Answer to relevant QuestionsWhat is an advantage of using an ELISA in-stead of a protein microarray to study a proteome? What is a disad-vantage? What are some of the assumptions behind the logic of the experiment described in figure 5.26? Why do most people elute bound proteins from an ion-exchange column by raising the salt concentration instead of changing the pH? What is meant by “salting out”? How does it work? Why is it useful to plot rate data for enzymatic reactions as a straight line rather than as a curve?
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