Why do most people elute bound proteins from an ion-exchange column by raising the salt concentration instead of changing the pH?
Answer to relevant QuestionsHow can gel-filtration chromatography be used to arrive at an estimate of the molecular weight of a protein? You wish to separate and purify enzyme A from contaminating enzymes B and C. Enzyme A is found in the matrix of the mitochondria. Enzyme B is embedded in the mito-chondrial membrane, and enzyme C is found in the peroxisome. ...For an enzyme that displays Michaelis–Menten kinetics, what is the reaction velocity, V (as a percentage of Vmax), observed at the following values? (a) [S] 5 KM (b) [S] 5 0.5KM (c) [S] 5 0.1KM (d) [S] 5 2KM (e) [S] 5 10KM Distinguish between the molecular mechanisms of competitive and noncompetitive inhibition. Is it good (or bad) that enzymes can be reversibly inhibited? Why?
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