Why is the order of separation based on size opposite for gel filtration and gel electrophoresis, even though they often use the same compound to form the matrix?
Answer to relevant QuestionsShow by a series of equations (with structures) the first stage of the Edman method applied to a peptide that has leucine as its N-terminal residue. Which enzymes or chemicals would you choose to use to cut the protein from Question 50? Why? What is the advantage to transferring proteins bands form a gel to nitrocellulose during the western blot? What is the basis for the separation of proteins by the following techniques? (a) Gel-filtration chromatography (b) Affinity chromatography (c) Ion-exchange chromatography (d) Reverse phase HPLC You wish to separate and purify enzyme A from contaminating enzymes B and C. Enzyme A is found in the matrix of the mitochondria. Enzyme B is embedded in the mito-chondrial membrane, and enzyme C is found in the peroxisome. ...
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