You are interested in discerning the role of Ï factors in prokaryotic transcription, using a system of

You are interested in discerning the role of σ factors in prokaryotic transcription, using a
system of purified core polymerase, a DNA template, a σ factor, and labeled nucleotides
(32pppN). The incorporation of 32P into RNA at 2 different core enzyme concentrations (10-
10 M and 10-11 M) and constant concentration of σ factor (10-12 M) is shown below.
(a) What does the graph measure?
(b) What can you conclude about the behavior of σ factor?
(c) What can you conclude about the behavior of the core enzyme?

Transcribed Image Text:

1010M core enzyme 10M core enzyme 10 ime (mins)