Answer the following questions that pertain to the experiment of Figure 14.7. A. Why was P-ONPG used?

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Answer the following questions that pertain to the experiment of Figure 14.7.
A. Why was P-ONPG used? Why was no yellow color observed in one of the four tubes? Can you propose alternative methods to measure the level of expression of the lac operon?
B. The optical density values were twice as high for the mated strain as for the parent strain. Why was this result obtained?
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