DNA isolated from an organism can be sheared into fragments of uniform size (1000 bp), heated to

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DNA isolated from an organism can be sheared into fragments of uniform size (1000 bp), heated to separate the strands, then cooled to allow complementary strands to reanneal. The renaturation process can be followed over time. Explain why the renaturation of E. coli DNA is a monophasic process whereas the renaturation of human DNA is biphasic (an initial rapid phase followed by a slower phase).
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Fundamentals of biochemistry Life at the Molecular Level

ISBN: 978-0470547847

4th edition

Authors: Donald Voet, Judith G. Voet, Charlotte W. Pratt

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