A mixture of three amino aids is separated by gel electrophoresis. The three amino acids are glycine, valine and phenylalanine (Figure 27.14).

a. The electrophoresis is carried out in a buffer solution of pH 10. Draw the ions present in these alkaline conditions.

b. i. Draw a sketch of the electropherogram you would expect (viewed from above), labelling the amino acids as Gly, Val and Phe.

ii. Explain your answer to part b i.

Transcribed Image Text:

H,N- CH -COOH H,N-CH-COOH H CHCH3 CH; valine (Val) glycine (Gly) H,N- CH- COOH CH2 phenylalanine (Phe) Figure 27.14 Three amino acids to be separated by electrophoresis.