If isolated mitochondria are incubated with a source of electrons such as succinate, but without oxygen, electrons enter the respiratory chain, reducing each of the electron carriers almost completely. When oxygen is then introduced, the carriers become oxidized at different rates (Figure Q14–1). How does this result allow you to order the electron carriers in the respiratory chain? What is their order?

Figure Q14-1

Transcribed Image Text:

100 add O2 50 add succinate (a + az) time reduced cytochrome (percent)