In the very first round of PCR using genomic DNA, the DNA primers prime synthesis that terminates
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In the very first round of PCR using genomic DNA, the DNA primers prime synthesis that terminates only when the cycle ends (or when a random end of DNA is encountered). Yet, by the end of 20 to 30 cycles—a typical amplification—the only visible product is defined precisely by the ends of the DNA primers. In what cycle is a double-stranded fragment of the correct size first generated?
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Related Book For
Molecular Biology Of The Cell
ISBN: 9780815344322
6th Edition
Authors: Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
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