What does the budding of influenza virus and vesicular stomatitis virus (VSV) from polarized MDCK cells reveal
Question:
What does the budding of influenza virus and vesicular stomatitis virus (VSV) from polarized MDCK cells reveal about the sorting of newly synthesized cell plasma membrane proteins to the apical or basolateral domains? Now consider the following result: A peptide with a sequence identical to that of the VSV G protein cytoplasmic domain inhibits targeting of the G protein to the basolateral surface and has no effect on HA targeting to the apical membrane, but a peptide in which the single tyrosine residue is mutated to an alanine has no effect on G protein basolateral targeting. What does this tell you about the sorting process?
Step by Step Answer:
Molecular Cell Biology
ISBN: 978-1429234139
7th edition
Authors: Harvey Lodish, Arnold Berk, Chris A. Kaiser, Monty Krieger, Anthony Bretscher, Hidde Ploegh, Angelika Amon, Matthew P. Scott