Green fluorescent protein (GFP), first isolated from bioluminescent jellyfish, is a protein containing 238 amino acid residues.

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Green fluorescent protein (GFP), first isolated from bioluminescent jellyfish, is a protein containing 238 amino acid residues. The discovery of GFP has revolutionized the field of fluorescence microscopy, which enables biochemists to monitor the biosynthesis of proteins. The 2008 Nobel Prize in Chemistry was awarded to Martin Chalfie, Osamu Shimomura, and Roger Tsien for the discovery and development of GFP. The structural subunit of GFP responsible for fluorescence, called the fluorophore, results when three amino acid residues undergo cyclization. Identify the three amino acids that go into the biosynthesis of this fluorophore:

НО. н N° Ň. -N- но

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Organic Chemistry

ISBN: 978-0470917800

1st edition

Authors: David R. Klein

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