In a FRET experiment designed to monitor conformational changes in T4 lysozyme, the fluorescence intensity fluctuates between
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In a FRET experiment designed to monitor conformational changes in T4 lysozyme, the fluorescence intensity fluctuates between 5000 and 10,000 counts per second. Assuming that 7500 counts represent a FRET efficiency of 0.5, what is the change in FRET pair separation distance during the reaction? For the tetramethylrhodamine/Texas red FRET pair employed, r0 = 50.Å.
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