E. coli cells that have been growing in 14 N for many generations are transferred to medium

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E. coli cells that have been growing in 14N for many generations are transferred to medium containing only 15N and allowed to grow in this medium for four generations. Their DNA is then extracted and analyzed by equilibrium CsCl density-gradient centrifugation. What proportion of this DNA will band at the “light,” “hybrid,” and “heavy” positions in the gradient? 

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Principles of Genetics

ISBN: 978-1119142287

7th edition

Authors: D. Peter Snustad, Michael J. Simmons

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