In the following illustration, the upper line shows a gene composed of segments AD. The lower circle
Question:
You prepare DNA from the cells, digest it with an enzyme that cuts at x, and hybridize the cleaved DNA with the probe shown above. The following diagram shows a Southern blot of possible results.
(a) Which lane shows fragments produced from DNA in the cell before transformation?
(b) Which lane shows fragments produced from DNA in the cell in which the anticipated targeted mutagenesis occurred?
(c) Which of these blot patterns might be expected if two crossovers occurred, one between A and B, and the other between C and D?
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Principles of Genetics
ISBN: 978-1119142287
7th edition
Authors: D. Peter Snustad, Michael J. Simmons
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