Suppose that you used site-specific mutagenesis to modify the trpL sequence such that the two UGG Trp
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Suppose that you used site-specific mutagenesis to modify the trpL sequence such that the two UGG Trp codons at positions 54–56 and 57–60 (Figure 17.14) in the mRNA leader sequence were changed to GGG Gly codons. Will attenuation of the trp operon still be regulated by the presence or absence of tryptophan in the medium in which the E. coli cells are growing?
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Related Book For
Principles of Genetics
ISBN: 978-1119142287
7th edition
Authors: D. Peter Snustad, Michael J. Simmons
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