The drawing in this problem shows a restriction map of a segment of a DNA molecule. Eco refers to locations where the restriction endonuclease EcoRI cuts the DNA, and Pst refers to locations where the restriction enzyme PstI cuts the DNA. Potential restriction sites are numbered 1€“6. Distances between restriction sites are shown on the bottom scale in base pairs (bp). The thick line represents the part of the molecule that has homology with a probe.

(a) Assume that individual 1 has restriction sites 1 through 6. If this individual€™s DNA is digested with PstI, what are the expected sizes of the DNA fragments that will hybridize with the probe?

(b) Assume that individual 2 has a mutation that eliminates site 4. If this individual€™s DNA is digested with PstI, what are the expected sizes of the DNA fragments that will hybridize with the probe? 

(c) Assume that individual 3 has a mutation that eliminates site 5. If the DNA of this individual is digested with PstI, what are the expected sizes of the DNA fragments that will hybridize with the probe?

(d) If the DNA of individual 1 is digested with both PstI and EcoRI, what are the expected sizes of the DNA fragments that will hybridize with the probe? 

(e) If the DNA of individual 3 is digested with both PstI and EcoRI, what are the expected sizes of the DNA fragments that will hybridize with the probe?

Transcribed Image Text:

Eco Eco Pst Pst Eco Pst 2 5000 bp 4 2000 bp 3 5000 bp 3000 bp 4000 bp