Total RNA was isolated from human cells growing in culture. This RNA was mixed with nontemplate strands

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Total RNA was isolated from human cells growing in culture. This RNA was mixed with nontemplate strands (single strands) of the human gene encoding the enzyme thymidine kinase, and the RNA–DNA mixture was incubated for 12 hours under renaturation conditions. Would you expect any RNA–DNA duplexes to be formed during the incubation? If so, why? If not, why not? The same experiment was then performed using the template strand of the thymidine kinase gene. Would you expect any RNA–DNA duplexes to be formed in this second experiment? If so, why? If not, why not?

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Principles of Genetics

ISBN: 978-1119142287

7th edition

Authors: D. Peter Snustad, Michael J. Simmons

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