You are working as part of a research team studying the structure and function of a particular gene. Your job is to clone the gene. A restriction map is available for the region of the chromosome in which the gene is located; the map is as follows:

Your first task is to prepare a genomic DNA library that contains clones carrying the entire gene. Describe how you would prepare such a library in plasmid vector p-Blue script II, indicating which restriction enzymes, media, and host cells you would use.

Transcribed Image Text:

Hindll Sall Xbal Pstl EcoRI EcoRI Hindll GENE