Signal in attenuated total reflectance is related to penetration depth.
a. Calculate the penetration depth of 1 500 cm^-1 1500 cm^-1 radiation into an organic sample (n2=1.5)(n₂=1.5) for a 45°450° angle of incidence with a diamond waveguide (n1=2.42).(n₁=2.42).
b. Calculate the penetration depth of 3 000 cm^-1 3 000 cm^-1 radiation under the same experimental conditions.
c. It is difficult to compare attenuated total reflectance spectra with transmission spectra because relative intensities of bands are different. Based on (a) and (b), why are the relative intensities of bands different?
d. Attenuated total reflectance is not used with visible or ultraviolet radiation. Calculate the depth of penetration of 500- nm 500- nm light to determine why.