Restriction endonuclease digestions typically use 1U of ezyme per g of DNA. 1U is defined as the
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Question:
Restriction endonuclease digestions typically use 1U of ezyme per µg of DNA. 1U is defined as the amount of enzyme required to completely digest 1 µg of DNA in 1 hour. What would happen if only 0.05U were used to digest 10 µg of DNA?
A. The degree of digestion depends on the methylation state of the recognition sequence.
B. The degree of digestion depends on the star activity of the enzyme.
C. The DNA would be partially digested after 1 hour incubation.
D. The DNA would be undigested after 1 hour incubation.
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