The following table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents...
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The following table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme EcoRI EcoRV Haelll Hindill PpuMI Target sequence 5' GAATTC 3' 3' CTTAAG 5' Haelll EcoRV EcoRI Hindill 5' GATATC 3' 3' CTATAG 5' 5' GGCC 3' 3' CCGG 5' 5' AAGCTT 3' 3' TTCGAA 5' 5' RGGWCCY 3' 3' YCCWGGR 5' Cleavage 5' G 3' CTTAA 5' GAT 3' CTA 5' GG 3' CC ATC 3' TAG 5' AATTC 3' G 5' CC 3' GG 5' 5' A 3' TTCGA 5' RG 3' YCCWG AGCTT 3' A 5' GWCCY 3' GR 5' Which restriction endonuclease(s) would cleave a DNA molecule at the following sequences? Note: The complementary DNA substrate strand is omitted for clarity. a) 5' TCAGAATTCGGTGA 3' b) 5' AGGCCGAATTCGC 3' Haelll EcoRI EcoRV Hindill The following table shows where different restriction endonucleases (restriction enzymes) cleave DNA. The abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine. Enzyme EcoRI EcoRV Haelll Hindill PpuMI Target sequence 5' GAATTC 3' 3' CTTAAG 5' Haelll EcoRV EcoRI Hindill 5' GATATC 3' 3' CTATAG 5' 5' GGCC 3' 3' CCGG 5' 5' AAGCTT 3' 3' TTCGAA 5' 5' RGGWCCY 3' 3' YCCWGGR 5' Cleavage 5' G 3' CTTAA 5' GAT 3' CTA 5' GG 3' CC ATC 3' TAG 5' AATTC 3' G 5' CC 3' GG 5' 5' A 3' TTCGA 5' RG 3' YCCWG AGCTT 3' A 5' GWCCY 3' GR 5' Which restriction endonuclease(s) would cleave a DNA molecule at the following sequences? Note: The complementary DNA substrate strand is omitted for clarity. a) 5' TCAGAATTCGGTGA 3' b) 5' AGGCCGAATTCGC 3' Haelll EcoRI EcoRV Hindill
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Related Book For
Stats Data and Models
ISBN: 978-0321986498
4th edition
Authors: Richard D. De Veaux, Paul D. Velleman, David E. Bock
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